Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1240067..1240904 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag HPE50_RS05965 Protein ID WP_000227787.1
Coordinates 1240067..1240609 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag HPE50_RS05970 Protein ID WP_001353405.1
Coordinates 1240593..1240904 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS05945 1235607..1236518 - 912 WP_001571020.1 2-dehydropantoate 2-reductase -
HPE50_RS05950 1236686..1237177 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HPE50_RS05955 1237305..1238669 - 1365 WP_001000966.1 MFS transporter -
HPE50_RS05960 1239076..1240011 + 936 WP_001571021.1 sel1 repeat family protein -
HPE50_RS05965 1240067..1240609 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
HPE50_RS05970 1240593..1240904 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
HPE50_RS05975 1241089..1241979 - 891 WP_000971328.1 heme o synthase -
HPE50_RS05980 1241991..1242320 - 330 WP_000019803.1 cytochrome o ubiquinol oxidase subunit IV -
HPE50_RS05985 1242320..1242934 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HPE50_RS05990 1242924..1244915 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HPE50_RS05995 1244937..1245884 - 948 WP_001571023.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T159439 WP_000227787.1 NZ_CP054353:c1240609-1240067 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T159439 NZ_CP070046:c1931334-1930966 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT159439 WP_001353405.1 NZ_CP054353:c1240904-1240593 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT159439 NZ_CP070046:c1931552-1931331 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References