Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 477476..477973 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HPE50_RS02190 Protein ID WP_001173343.1
Coordinates 477800..477973 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HPE50_RS02185 Protein ID WP_000398619.1
Coordinates 477476..477772 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS02165 474205..474924 - 720 WP_000611288.1 two-component system response regulator DcuR -
HPE50_RS02170 474921..476552 - 1632 WP_001216448.1 sensor histidine kinase -
HPE50_RS02175 476733..476963 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
HPE50_RS02180 476975..477247 + 273 WP_000405647.1 N-acetyltransferase -
HPE50_RS02185 477476..477772 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HPE50_RS02190 477800..477973 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HPE50_RS02195 478092..479609 - 1518 WP_000003806.1 lysine--tRNA ligase -
HPE50_RS02200 479846..481303 - 1458 WP_000856813.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T159434 WP_001173343.1 NZ_CP054353:477800-477973 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T159434 NZ_CP070045:c5186272-5186120 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT159434 WP_000398619.1 NZ_CP054353:477476-477772 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT159434 NZ_CP070045:5186321-5186378 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References