159433

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 8227..8752 Replicon plasmid pSCU-176-1
Accession NZ_CP054346
Organism Escherichia coli strain SCU-176

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HPE52_RS24240 Protein ID WP_001159871.1
Coordinates 8447..8752 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HPE52_RS24235 Protein ID WP_000813634.1
Coordinates 8227..8445 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE52_RS24210 3258..4274 - 1017 WP_001310555.1 IS5-like element IS5 family transposase -
HPE52_RS24215 4307..5329 - 1023 WP_000361402.1 helicase UvrD -
HPE52_RS24220 5314..6879 - 1566 WP_115766545.1 AAA family ATPase -
HPE52_RS24225 6954..7370 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HPE52_RS24230 7367..7597 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HPE52_RS24235 8227..8445 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HPE52_RS24240 8447..8752 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HPE52_RS24245 8753..9562 + 810 WP_000016979.1 site-specific integrase -
HPE52_RS24250 9700..9975 - 276 WP_000239529.1 hypothetical protein -
HPE52_RS24255 9969..10613 - 645 WP_000633911.1 AAA family ATPase -
HPE52_RS24260 10842..11813 + 972 WP_001103694.1 hypothetical protein -
HPE52_RS24265 11818..12210 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
HPE52_RS24270 12215..13038 - 824 Protein_16 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - afaE-V 1..39154 39154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T159433 WP_001159871.1 NZ_CP054346:8447-8752 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T159433 NZ_CP070045:5000925-5001302 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT159433 WP_000813634.1 NZ_CP054346:8227-8445 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT159433 NZ_CP070045:5000467-5000835 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References