159432

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6954..7597 Replicon plasmid pSCU-176-1
Accession NZ_CP054346
Organism Escherichia coli strain SCU-176

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HPE52_RS24225 Protein ID WP_001034044.1
Coordinates 6954..7370 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HPE52_RS24230 Protein ID WP_001261286.1
Coordinates 7367..7597 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE52_RS24200 2156..2797 - 642 Protein_2 IS66-like element ISEc22 family transposase -
HPE52_RS24205 2801..3075 - 275 Protein_3 transposase -
HPE52_RS24210 3258..4274 - 1017 WP_001310555.1 IS5-like element IS5 family transposase -
HPE52_RS24215 4307..5329 - 1023 WP_000361402.1 helicase UvrD -
HPE52_RS24220 5314..6879 - 1566 WP_115766545.1 AAA family ATPase -
HPE52_RS24225 6954..7370 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HPE52_RS24230 7367..7597 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HPE52_RS24235 8227..8445 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HPE52_RS24240 8447..8752 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
HPE52_RS24245 8753..9562 + 810 WP_000016979.1 site-specific integrase -
HPE52_RS24250 9700..9975 - 276 WP_000239529.1 hypothetical protein -
HPE52_RS24255 9969..10613 - 645 WP_000633911.1 AAA family ATPase -
HPE52_RS24260 10842..11813 + 972 WP_001103694.1 hypothetical protein -
HPE52_RS24265 11818..12210 + 393 WP_000340835.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - afaE-V 1..39154 39154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T159432 WP_001034044.1 NZ_CP054346:c7370-6954 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T159432 NZ_CP070045:4728347-4728520 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT159432 WP_001261286.1 NZ_CP054346:c7597-7367 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT159432 NZ_CP070045:4728023-4728319 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References