Detailed information of TA system TA159430

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4728073..4728907
• Replicon: chromosome
• Accession: NZ_CP054345
• Organism: Escherichia coli strain SCU-176

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IWF2
• Locus tag: HPE52_RS22770
• Protein ID: WP_001521667.1
• Coordinates: 4728530..4728907 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: HPE52_RS22765
• Protein ID: WP_001285610.1
• Coordinates: 4728073..4728441 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE52_RS22730	4723956..4724636	+	681	WP_073313349.1	WYL domain-containing protein	-
HPE52_RS22735	4724784..4725461	+	678	WP_174165906.1	hypothetical protein	-
HPE52_RS22740	4725467..4725700	+	234	WP_001521659.1	DUF905 family protein	-
HPE52_RS22745	4725799..4726617	+	819	WP_174165900.1	DUF945 domain-containing protein	-
HPE52_RS22750	4726709..4727194	+	486	WP_021524627.1	antirestriction protein	-
HPE52_RS22755	4727210..4727686	+	477	WP_001186724.1	RadC family protein	-
HPE52_RS22760	4727772..4727993	+	222	WP_001521666.1	DUF987 domain-containing protein	-
HPE52_RS22765	4728073..4728441	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HPE52_RS22770	4728530..4728907	+	378	WP_001521667.1	TA system toxin CbtA family protein	Toxin
HPE52_RS22775	4728904..4729053	+	150	Protein_4454	hypothetical protein	-
HPE52_RS22780	4729129..4729326	+	198	WP_086795284.1	DUF957 domain-containing protein	-
HPE52_RS22785	4729411..4730253	+	843	WP_001521669.1	DUF4942 domain-containing protein	-
HPE52_RS22790	4730849..4731346	+	498	WP_097430550.1	hypothetical protein	-
HPE52_RS22795	4731513..4732436	+	924	WP_097430551.1	carboxylate/amino acid/amine transporter	-
HPE52_RS22800	4732440..4733258	-	819	WP_000779409.1	lipoprotein NlpA	-
HPE52_RS22805	4733480..4733773	+	294	WP_001297978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4711105..4731346	20241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14035.97 Da
• Isoelectric Point: 6.8607

>T159430 WP_001521667.1 NZ_CP054345:4728530-4728907 [Escherichia coli]
MKTLPVLPGQAASSRPSSVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T159430 NZ_CP070045:c4031607-4031500 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT159430 WP_001285610.1 NZ_CP054345:4728073-4728441 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT159430 NZ_CP070045:4031664-4031721 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT