Detailed information of TA system TA159427

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3815377..3816212
• Replicon: chromosome
• Accession: NZ_CP054345
• Organism: Escherichia coli strain SCU-176

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: HPE52_RS18375
• Protein ID: WP_000854759.1
• Coordinates: 3815377..3815754 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HPE52_RS18380
• Protein ID: WP_001295723.1
• Coordinates: 3815844..3816212 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE52_RS18340	3811005..3811751	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
HPE52_RS18345	3811766..3813307	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
HPE52_RS18350	3813901..3814077	-	177	Protein_3590	helix-turn-helix domain-containing protein	-
HPE52_RS18355	3814441..3814599	-	159	WP_001467148.1	hypothetical protein	-
HPE52_RS18360	3814591..3814781	-	191	Protein_3592	hypothetical protein	-
HPE52_RS18365	3814699..3814875	-	177	WP_000839286.1	DUF957 domain-containing protein	-
HPE52_RS18370	3814892..3815380	-	489	WP_000761690.1	hypothetical protein	-
HPE52_RS18375	3815377..3815754	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
HPE52_RS18380	3815844..3816212	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HPE52_RS18385	3816375..3816596	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HPE52_RS18390	3816659..3817135	-	477	WP_001186775.1	RadC family protein	-
HPE52_RS18395	3817151..3817624	-	474	WP_001350782.1	antirestriction protein	-
HPE52_RS18400	3817719..3817966	-	248	Protein_3600	antirestriction protein	-
HPE52_RS18405	3817966..3818784	-	819	WP_001234738.1	DUF945 domain-containing protein	-
HPE52_RS18410	3818939..3819097	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3792454..3830405	37951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T159427 WP_000854759.1 NZ_CP054345:c3815754-3815377 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T159427 NZ_CP070045:c3323591-3323184 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT159427 WP_001295723.1 NZ_CP054345:c3816212-3815844 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT159427 NZ_CP070045:c3323838-3323572 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52