159423

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3641206..3641756 Replicon chromosome
Accession NZ_CP054345
Organism Escherichia coli strain SCU-176

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HPE52_RS17525 Protein ID WP_001160966.1
Coordinates 3641206..3641520 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag HPE52_RS17530 Protein ID WP_000125568.1
Coordinates 3641523..3641756 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE52_RS17500 3636881..3638167 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HPE52_RS17505 3638167..3639156 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HPE52_RS17510 3639153..3639974 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HPE52_RS17515 3639977..3640354 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HPE52_RS17520 3640361..3641209 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HPE52_RS17525 3641206..3641520 - 315 WP_001160966.1 CcdB family protein Toxin
HPE52_RS17530 3641523..3641756 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HPE52_RS17535 3641842..3642321 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HPE52_RS17540 3642513..3644375 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
HPE52_RS17545 3644368..3644898 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HPE52_RS17550 3645006..3646337 - 1332 WP_001183207.1 MFS transporter -
HPE52_RS17555 3646396..3646683 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T159423 WP_001160966.1 NZ_CP054345:c3641520-3641206 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T159423 NZ_CP070045:2240546-2240920 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT159423 WP_000125568.1 NZ_CP054345:c3641756-3641523 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT159423 NZ_CP070045:2240089-2240457 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References