Detailed information of TA system TA159396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 170207..170943
• Replicon: plasmid pMS14393A
• Accession: NZ_CP054304
• Organism: Klebsiella pneumoniae strain MS14393

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: MS14393_RS27760
• Protein ID: WP_003026803.1
• Coordinates: 170461..170943 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MS14393_RS27755
• Protein ID: WP_003026799.1
• Coordinates: 170207..170473 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS14393_RS27710	166269..166631	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
MS14393_RS27715	166681..167031	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
MS14393_RS27720	167389..167658	+	270	WP_004152102.1	hypothetical protein	-
MS14393_RS27725	167646..168221	+	576	WP_004152103.1	hypothetical protein	-
MS14393_RS27730	168252..168746	+	495	WP_004152104.1	DNA-binding protein	-
MS14393_RS27735	168790..169158	+	369	WP_004152105.1	hypothetical protein	-
MS14393_RS27740	169192..169395	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
MS14393_RS27745	169444..169701	+	258	WP_004152107.1	hypothetical protein	-
MS14393_RS27750	169777..170031	+	255	WP_004152108.1	hypothetical protein	-
MS14393_RS27755	170207..170473	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MS14393_RS27760	170461..170943	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
MS14393_RS27765	171102..172356	-	1255	Protein_177	IS3 family transposase	-
MS14393_RS27770	172444..173406	-	963	WP_004152113.1	M48 family metalloprotease	-
MS14393_RS27775	173393..174142	-	750	WP_009483782.1	phosphate-starvation-inducible PsiE family protein	-
MS14393_RS27780	174380..174577	-	198	WP_004152115.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..216803	216803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T159396 WP_003026803.1 NZ_CP054304:170461-170943 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T159396 NZ_CP070041:751467-751745 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT159396 WP_003026799.1 NZ_CP054304:170207-170473 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT159396 NZ_CP070041:751745-752029 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66