Detailed information of TA system TA159392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4540053..4540678
• Replicon: chromosome
• Accession: NZ_CP054303
• Organism: Klebsiella pneumoniae strain MS14393

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MS14393_RS22455
• Protein ID: WP_021312635.1
• Coordinates: 4540295..4540678 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: MS14393_RS22450
• Protein ID: WP_004150355.1
• Coordinates: 4540053..4540295 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS14393_RS22425	4536044..4536643	+	600	WP_040148118.1	glucose-1-phosphatase	-
MS14393_RS22430	4536637..4537497	+	861	WP_004146232.1	virulence factor BrkB family protein	-
MS14393_RS22435	4537494..4537931	+	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MS14393_RS22440	4537976..4538917	+	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
MS14393_RS22445	4538931..4539848	-	918	WP_004178029.1	alpha/beta hydrolase	-
MS14393_RS22450	4540053..4540295	+	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
MS14393_RS22455	4540295..4540678	+	384	WP_021312635.1	type II toxin-antitoxin system VapC family toxin	Toxin
MS14393_RS22460	4540852..4541781	-	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
MS14393_RS22465	4541778..4542413	-	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
MS14393_RS22470	4542410..4543312	-	903	WP_002882822.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14349.56 Da
• Isoelectric Point: 7.3178

>T159392 WP_021312635.1 NZ_CP054303:4540295-4540678 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVATPYEIHPE

Nucleotide

• Length: 384 bp

>T159392 NZ_CP070041:81581-81835 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT159392 WP_004150355.1 NZ_CP054303:4540053-4540295 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT159392 NZ_CP070041:81333-81584 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9