Detailed information of TA system TA159369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 21246..21939
• Replicon: plasmid p1C157
• Accession: NZ_CP054295
• Organism: Citrobacter freundii strain IDR1800045912-01-00

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: HS089_RS24100
• Protein ID: WP_000182276.1
• Coordinates: 21583..21939 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: HS089_RS24095
• Protein ID: WP_001172026.1
• Coordinates: 21246..21581 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS089_RS24055	17077..17406	+	330	WP_000493286.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HS089_RS24060	17387..17668	+	282	WP_000780222.1	helix-turn-helix transcriptional regulator	-
HS089_RS24065	17946..18197	+	252	Protein_19	transposase	-
HS089_RS24070	18237..18632	-	396	WP_104455936.1	transposase	-
HS089_RS24075	18799..19914	-	1116	WP_171277333.1	HD-GYP domain-containing protein	-
HS089_RS24080	20169..20549	-	381	WP_001054412.1	hypothetical protein	-
HS089_RS24085	20546..20872	-	327	WP_000091614.1	hypothetical protein	-
HS089_RS24090	20896..21231	-	336	WP_000741275.1	hypothetical protein	-
HS089_RS24095	21246..21581	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
HS089_RS24100	21583..21939	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HS089_RS24105	22041..22112	+	72	Protein_27	EAL domain-containing protein	-
HS089_RS24110	22252..23016	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
HS089_RS24115	23134..23202	-	69	Protein_29	EAL domain-containing protein	-
HS089_RS24120	23523..24023	-	501	WP_000376623.1	GNAT family N-acetyltransferase	-
HS089_RS24125	24151..24990	-	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
HS089_RS24130	24984..25331	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
HS089_RS24135	25554..26006	-	453	WP_001749986.1	NAD(+)--rifampin ADP-ribosyltransferase Arr-3	-
HS089_RS24140	26091..26723	-	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / aac(3)-IId / mph(A) / sul2	-	1..156725	156725
	-	inside	IScluster/Tn	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / aac(3)-IId / mph(A) / sul2	-	10037..54381	44344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T159369 WP_000182276.1 NZ_CP054295:c21939-21583 [Citrobacter freundii]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T159369 NZ_CP070026:3311248-3311424 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT159369 WP_001172026.1 NZ_CP054295:c21581-21246 [Citrobacter freundii]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT159369 NZ_CP070026:3311470-3311886 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7