Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4799419..4800035 Replicon chromosome
Accession NZ_CP054294
Organism Citrobacter freundii strain IDR1800045912-01-00

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HS089_RS23065 Protein ID WP_003028682.1
Coordinates 4799419..4799793 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HS089_RS23070 Protein ID WP_043018956.1
Coordinates 4799793..4800035 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS089_RS23050 4796922..4797824 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HS089_RS23055 4797821..4798456 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HS089_RS23060 4798453..4799382 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HS089_RS23065 4799419..4799793 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HS089_RS23070 4799793..4800035 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HS089_RS23075 4800241..4801149 + 909 WP_044701816.1 alpha/beta hydrolase -
HS089_RS23080 4801300..4802241 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HS089_RS23085 4802286..4802723 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HS089_RS23090 4802720..4803592 - 873 WP_003028669.1 virulence factor BrkB family protein -
HS089_RS23095 4803586..4804185 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T159368 WP_003028682.1 NZ_CP054294:c4799793-4799419 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T159368 NZ_CP070026:c3221131-3220937 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT159368 WP_043018956.1 NZ_CP054294:c4800035-4799793 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT159368 NZ_CP070026:3220760-3220938 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References