Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3910476..3911155 Replicon chromosome
Accession NZ_CP054294
Organism Citrobacter freundii strain IDR1800045912-01-00

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HS089_RS18860 Protein ID WP_003031349.1
Coordinates 3910814..3911155 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HS089_RS18855 Protein ID WP_003031347.1
Coordinates 3910476..3910793 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS089_RS18830 3906569..3908566 - 1998 WP_016245729.1 choline BCCT transporter BetT -
HS089_RS18835 3909070..3909261 + 192 Protein_3691 DUF905 family protein -
HS089_RS18840 3909275..3909736 + 462 WP_003031344.1 antirestriction protein -
HS089_RS18845 3909752..3910228 + 477 WP_003031345.1 RadC family protein -
HS089_RS18850 3910237..3910458 + 222 WP_003031346.1 DUF987 domain-containing protein -
HS089_RS18855 3910476..3910793 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HS089_RS18860 3910814..3911155 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HS089_RS18865 3911745..3912236 - 492 WP_121927018.1 tail fiber assembly protein -
HS089_RS18870 3913357..3913800 - 444 Protein_3698 phage tail protein -
HS089_RS18875 3913800..3914480 - 681 WP_121927014.1 DUF2612 domain-containing protein -
HS089_RS18880 3914477..3915676 - 1200 WP_121927013.1 baseplate J/gp47 family protein -
HS089_RS18885 3915677..3916030 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3895519..3953671 58152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T159365 WP_003031349.1 NZ_CP054294:3910814-3911155 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T159365 NZ_CP070026:c3066923-3066816 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT159365 WP_003031347.1 NZ_CP054294:3910476-3910793 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT159365 NZ_CP070026:3066971-3067037 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References