Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3673674..3674294 Replicon chromosome
Accession NZ_CP054294
Organism Citrobacter freundii strain IDR1800045912-01-00

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HS089_RS17750 Protein ID WP_002892050.1
Coordinates 3674076..3674294 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HS089_RS17745 Protein ID WP_003021733.1
Coordinates 3673674..3674048 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS089_RS17735 3668821..3670014 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HS089_RS17740 3670037..3673186 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HS089_RS17745 3673674..3674048 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HS089_RS17750 3674076..3674294 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HS089_RS17755 3674601..3675152 + 552 WP_044699372.1 maltose O-acetyltransferase -
HS089_RS17760 3675269..3675739 + 471 WP_003021724.1 YlaC family protein -
HS089_RS17765 3675818..3675958 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HS089_RS17770 3675960..3676220 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HS089_RS17775 3676409..3677962 + 1554 WP_044699371.1 EAL domain-containing protein -
HS089_RS17780 3678014..3678367 - 354 WP_003831033.1 DUF1428 family protein -
HS089_RS17785 3678432..3679061 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159364 WP_002892050.1 NZ_CP054294:3674076-3674294 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T159364 NZ_CP070026:c2316635-2316417 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT159364 WP_003021733.1 NZ_CP054294:3673674-3674048 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT159364 NZ_CP070026:c2317035-2316661 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References