Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 31059..31795 Replicon plasmid p3K176
Accession NZ_CP054286
Organism Klebsiella pneumoniae strain IDR1800050501-01-02

Toxin (Protein)


Gene name tacT Uniprot ID A0A486JQ35
Locus tag HS091_RS25720 Protein ID WP_049109282.1
Coordinates 31059..31541 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HS091_RS25725 Protein ID WP_003026799.1
Coordinates 31529..31795 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS091_RS25700 26531..26938 - 408 WP_023304028.1 hypothetical protein -
HS091_RS25705 27082..28617 + 1536 Protein_25 IS3 family transposase -
HS091_RS25710 28794..29427 + 634 Protein_26 hypothetical protein -
HS091_RS25715 29456..30859 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HS091_RS25720 31059..31541 - 483 WP_049109282.1 GNAT family N-acetyltransferase Toxin
HS091_RS25725 31529..31795 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HS091_RS25730 32081..32188 + 108 Protein_30 transposase -
HS091_RS25735 32231..33046 - 816 WP_049109278.1 hypothetical protein -
HS091_RS25740 33401..34105 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HS091_RS25745 34157..34441 + 285 Protein_33 ISNCY family transposase -
HS091_RS25750 35489..36766 - 1278 WP_023307507.1 DUF2254 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..176330 176330
- inside IScluster/Tn - - 27580..34462 6882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17336.86 Da        Isoelectric Point: 8.0020

>T159350 WP_049109282.1 NZ_CP054286:c31541-31059 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCETGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T159350 NZ_CP070020:c1278174-1277956 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT159350 WP_003026799.1 NZ_CP054286:c31795-31529 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT159350 NZ_CP070020:c1278575-1278201 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486JQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References