Detailed information of TA system TA159349

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 5241549..5242275
• Replicon: chromosome
• Accession: NZ_CP054285
• Organism: Klebsiella pneumoniae strain IDR1800050501-01-02

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HS091_RS25475
• Protein ID: WP_107357767.1
• Coordinates: 5241549..5241890 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HS091_RS25480
• Protein ID: WP_107357768.1
• Coordinates: 5241925..5242275 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS091_RS25445	5236567..5236833	+	267	WP_004178415.1	hypothetical protein	-
HS091_RS25450	5236833..5237505	+	673	Protein_4966	DUF4400 domain-containing protein	-
HS091_RS25455	5237516..5238400	+	885	WP_004192285.1	RES domain-containing protein	-
HS091_RS25460	5238599..5238787	-	189	Protein_4968	transposase	-
HS091_RS25465	5238804..5239915	-	1112	Protein_4969	IS3 family transposase	-
HS091_RS25470	5240283..5241290	-	1008	WP_107357766.1	restriction endonuclease	-
HS091_RS25475	5241549..5241890	-	342	WP_107357767.1	TA system toxin CbtA family protein	Toxin
HS091_RS25480	5241925..5242275	-	351	WP_107357768.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HS091_RS25485	5242296..5242517	-	222	WP_107357769.1	DUF987 domain-containing protein	-
HS091_RS25490	5242533..5243006	-	474	WP_107357770.1	DNA repair protein RadC	-
HS091_RS25495	5243077..5243898	-	822	WP_032437350.1	DUF945 domain-containing protein	-
HS091_RS25500	5243994..5244671	-	678	WP_107357771.1	hypothetical protein	-
HS091_RS25505	5244956..5245849	-	894	WP_107357772.1	50S ribosome-binding GTPase	-
HS091_RS25510	5245948..5247045	-	1098	WP_117031494.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5230678..5266107	35429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (15-115)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12929.02 Da
• Isoelectric Point: 10.0932

>T159349 WP_107357767.1 NZ_CP054285:c5241890-5241549 [Klebsiella pneumoniae]
MNTLPAINQRAVQTCLSPVVVWQMLLARLLEQHYGLTLNDTPFSDEAVIKRHIEAGITLVDAVNFLLEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T159349 NZ_CP070020:c1033340-1033008 [Klebsiella oxytoca]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGACATGCTTATCACCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGACTACTTGAGCAGCACTATGGCCTGACCTTAAATGACACGCCGTTCAGCGATGAGGCTGTTCTTAAAAGACATATCG
AAGCTGGTATCACGCTTGTCGATGCCGTTAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGATCGCAGAGGATTT
AGCTCACAGGGACAAGTGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGAATGTGGATTAACGAACAGTTG
TCCTTATCGGTAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 12974.90 Da
• Isoelectric Point: 6.6206

>AT159349 WP_107357768.1 NZ_CP054285:c5242275-5241925 [Klebsiella pneumoniae]
MSNKTLTVNDDTAEPWWGLSRNVIPCFGARLVQEGNRLHYLAGRASLTGQIHEADLLHLDQAFPVLLKQMELMLTCGELN
PRHQHCVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 351 bp

>AT159349 NZ_CP070020:c1033725-1033375 [Klebsiella oxytoca]
ATGTCGAGTAAGACACTCACAGTGAATGATGATACCGCTGAGCCCTGGTGGGGGCTGAATCGTAATGTCATTCCTTGTTT
TGGTGCGCGTCTGGTCCAGGAAGGTAACCGCCTATATTACCTGTCTGACCGCGCCAGCATAATAGGCCTATTCAATAAAG
CGGATTTGCTCCGTCTTGACCAGGCATTTCCTGTTCTGCTGAAGCAGGCGGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACCCTCTATGCAAAGGGGCTGACCTGCGAAGCTGATACACTGGGTTCCTGTGGTCACGT
CTATATCTCCATTTATCCTACTCAACGCTAA