Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5201750..5202560 Replicon chromosome
Accession NZ_CP054285
Organism Klebsiella pneumoniae strain IDR1800050501-01-02

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag HS091_RS25265 Protein ID WP_023279404.1
Coordinates 5201750..5202283 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HS091_RS25270 Protein ID WP_002887278.1
Coordinates 5202294..5202560 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS091_RS25260 5200581..5201702 + 1122 WP_002887282.1 cupin domain-containing protein -
HS091_RS25265 5201750..5202283 - 534 WP_023279404.1 GNAT family N-acetyltransferase Toxin
HS091_RS25270 5202294..5202560 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HS091_RS25275 5202663..5204096 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
HS091_RS25280 5204086..5204769 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HS091_RS25285 5204941..5206326 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
HS091_RS25290 5206344..5206688 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T159348 WP_023279404.1 NZ_CP054285:c5202283-5201750 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T159348 NZ_CP070020:1017806-1018063 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGTGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAATGGGCAACCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT159348 WP_002887278.1 NZ_CP054285:c5202560-5202294 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT159348 NZ_CP070020:1018074-1018625 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGCTTTGCTCAACGATGAAAGTGCTCACCCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCCGTTGGGCATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCGCTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References