Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4307421..4308046 Replicon chromosome
Accession NZ_CP054282
Organism Escherichia coli strain IDR1900005638-01-02

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HS093_RS20475 Protein ID WP_000911329.1
Coordinates 4307648..4308046 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HS093_RS20470 Protein ID WP_000450524.1
Coordinates 4307421..4307648 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS093_RS20445 4303223..4303693 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HS093_RS20450 4303693..4304265 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HS093_RS20455 4304411..4305289 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HS093_RS20460 4305306..4306340 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HS093_RS20465 4306553..4307266 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HS093_RS20470 4307421..4307648 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HS093_RS20475 4307648..4308046 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HS093_RS20480 4308193..4309056 + 864 WP_001267498.1 neutral zinc metallopeptidase -
HS093_RS20485 4309071..4311086 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
HS093_RS20490 4311160..4311858 + 699 WP_000679823.1 esterase -
HS093_RS20495 4311968..4312168 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T159330 WP_000911329.1 NZ_CP054282:4307648-4308046 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T159330 NZ_CP070008:c113778-113473 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT159330 WP_000450524.1 NZ_CP054282:4307421-4307648 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT159330 NZ_CP070008:c113998-113780 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References