Detailed information of TA system TA159322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1737176..1738013
• Replicon: chromosome
• Accession: NZ_CP054282
• Organism: Escherichia coli strain IDR1900005638-01-02

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HS093_RS08300
• Protein ID: WP_000227784.1
• Coordinates: 1737471..1738013 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HS093_RS08295
• Protein ID: WP_001297137.1
• Coordinates: 1737176..1737487 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS093_RS08270	1732196..1733143	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HS093_RS08275	1733165..1735156	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HS093_RS08280	1735146..1735760	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HS093_RS08285	1735760..1736089	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HS093_RS08290	1736101..1736991	+	891	WP_000971336.1	heme o synthase	-
HS093_RS08295	1737176..1737487	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HS093_RS08300	1737471..1738013	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HS093_RS08305	1738069..1739004	-	936	WP_001297127.1	sel1 repeat family protein	-
HS093_RS08310	1739412..1740776	+	1365	WP_001000978.1	MFS transporter	-
HS093_RS08315	1740904..1741395	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HS093_RS08320	1741563..1742474	+	912	WP_000705849.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T159322 WP_000227784.1 NZ_CP054282:1737471-1738013 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T159322 NZ_CP070007:4035590-4035693 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT159322 WP_001297137.1 NZ_CP054282:1737176-1737487 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT159322 NZ_CP070007:c4035731-4035460 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3