Detailed information of TA system TA159310

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4847111..4847790
• Replicon: chromosome
• Accession: NZ_CP054278
• Organism: Citrobacter freundii strain IDR1900015725-01-02

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: J7FU23
• Locus tag: HS092_RS23295
• Protein ID: WP_015063033.1
• Coordinates: 4847449..4847790 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HS092_RS23290
• Protein ID: WP_182936593.1
• Coordinates: 4847111..4847428 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS092_RS23270	4842840..4845035	+	2196	WP_035893672.1	DEAD/DEAH box helicase	-
HS092_RS23275	4845890..4846369	+	480	WP_015063037.1	antirestriction protein	-
HS092_RS23280	4846385..4846861	+	477	WP_015063036.1	RadC family protein	-
HS092_RS23285	4846871..4847092	+	222	WP_015063035.1	DUF987 domain-containing protein	-
HS092_RS23290	4847111..4847428	+	318	WP_182936593.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HS092_RS23295	4847449..4847790	+	342	WP_015063033.1	TA system toxin CbtA family protein	Toxin
HS092_RS23305	4848153..4848659	+	507	WP_071698087.1	G/U mismatch-specific DNA glycosylase	-
HS092_RS23310	4848690..4850687	-	1998	WP_003846276.1	TonB-dependent receptor	-
HS092_RS23315	4850698..4851747	-	1050	WP_182936594.1	YncE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4818940..4848659	29719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13051.09 Da
• Isoelectric Point: 9.9961

>T159310 WP_015063033.1 NZ_CP054278:4847449-4847790 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDTGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T159310 NZ_CP070007:396788-397006 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11865.56 Da
• Isoelectric Point: 6.6246

>AT159310 WP_182936593.1 NZ_CP054278:4847111-4847428 [Citrobacter freundii]
MSNITLGLQRDITPRLGVRLVQEGNRLHYLADRASITGKFSEAECLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT159310 NZ_CP070007:396388-396762 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: J7FU23