Detailed information of TA system TA159309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4420075..4420706
• Replicon: chromosome
• Accession: NZ_CP054278
• Organism: Citrobacter freundii strain IDR1900015725-01-02

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HS092_RS21120
• Protein ID: WP_182936573.1
• Coordinates: 4420075..4420350 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1MMS0
• Locus tag: HS092_RS21125
• Protein ID: WP_003837808.1
• Coordinates: 4420347..4420706 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS092_RS21110	4416126..4418873	+	2748	WP_048218800.1	ribosome-associated ATPase/putative transporter RbbA	-
HS092_RS21115	4418873..4419997	+	1125	WP_032950438.1	ABC transporter permease	-
HS092_RS21120	4420075..4420350	+	276	WP_182936573.1	type II toxin-antitoxin system HicA family toxin	Toxin
HS092_RS21125	4420347..4420706	+	360	WP_003837808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HS092_RS21130	4420819..4421220	-	402	WP_003023387.1	nickel-responsive transcriptional regulator NikR	-
HS092_RS21135	4421270..4421848	-	579	WP_071697729.1	4'-phosphopantetheinyl transferase AcpT	-
HS092_RS21140	4421911..4422960	-	1050	WP_003023393.1	AI-2E family transporter	-
HS092_RS21145	4423094..4424311	+	1218	WP_016151126.1	MFS transporter	-
HS092_RS21150	4424315..4424872	-	558	WP_003837816.1	DcrB family lipoprotein	-
HS092_RS21155	4424945..4425610	-	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10268.92 Da
• Isoelectric Point: 10.5885

>T159309 WP_182936573.1 NZ_CP054278:4420075-4420350 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGDEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T159309 NZ_CP069999:c70521-70246 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13198.98 Da
• Isoelectric Point: 4.5462

>AT159309 WP_003837808.1 NZ_CP054278:4420347-4420706 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT159309 NZ_CP069999:c70805-70521 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MMS0