Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3964157..3964773 Replicon chromosome
Accession NZ_CP054278
Organism Citrobacter freundii strain IDR1900015725-01-02

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HS092_RS19030 Protein ID WP_003028682.1
Coordinates 3964157..3964531 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HS092_RS19035 Protein ID WP_043018956.1
Coordinates 3964531..3964773 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS092_RS19015 3961660..3962562 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HS092_RS19020 3962559..3963194 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HS092_RS19025 3963191..3964120 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HS092_RS19030 3964157..3964531 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HS092_RS19035 3964531..3964773 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HS092_RS19040 3964979..3965887 + 909 WP_071697867.1 alpha/beta hydrolase -
HS092_RS19045 3966038..3966979 - 942 WP_182936560.1 fatty acid biosynthesis protein FabY -
HS092_RS19050 3967024..3967461 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HS092_RS19055 3967458..3968330 - 873 WP_003840437.1 virulence factor BrkB family protein -
HS092_RS19060 3968324..3968923 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T159308 WP_003028682.1 NZ_CP054278:c3964531-3964157 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T159308 NZ_CP069998:c28332-28054 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT159308 WP_043018956.1 NZ_CP054278:c3964773-3964531 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT159308 NZ_CP069998:c28601-28332 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References