Detailed information of TA system TA159306

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 3457238..3457956
• Replicon: chromosome
• Accession: NZ_CP054278
• Organism: Citrobacter freundii strain IDR1900015725-01-02

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HS092_RS16725
• Protein ID: WP_047715719.1
• Coordinates: 3457238..3457558 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HS092_RS16730
• Protein ID: WP_047715718.1
• Coordinates: 3457591..3457956 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS092_RS16705	3454025..3454387	+	363	WP_057101774.1	endoribonuclease SymE	-
HS092_RS16710	3454440..3455075	-	636	WP_003845570.1	HNH endonuclease	-
HS092_RS16715	3455216..3456088	+	873	WP_071698399.1	HNH endonuclease	-
HS092_RS16720	3456289..3457125	-	837	WP_099530923.1	DUF4942 domain-containing protein	-
HS092_RS16725	3457238..3457558	-	321	WP_047715719.1	TA system toxin CbtA family protein	Toxin
HS092_RS16730	3457591..3457956	-	366	WP_047715718.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HS092_RS16735	3457988..3458209	-	222	WP_047715716.1	DUF987 family protein	-
HS092_RS16740	3458218..3458688	-	471	WP_047715715.1	DNA repair protein RadC	-
HS092_RS16745	3458759..3459577	-	819	WP_047715712.1	DUF945 domain-containing protein	-
HS092_RS16750	3459677..3460354	-	678	WP_049016525.1	hypothetical protein	-
HS092_RS16755	3460642..3461535	-	894	WP_047715709.1	50S ribosome-binding GTPase	-
HS092_RS16760	3462026..3462658	+	633	WP_028016395.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-105)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12128.89 Da
• Isoelectric Point: 5.9559

>T159306 WP_047715719.1 NZ_CP054278:c3457558-3457238 [Citrobacter freundii]
MHISTIPATVLVASRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDSAIQEHIEAGITLADAVNFLVERYELIRTDRKGF
TWQEQTPFLTAIDILRARRATGLMNI

Nucleotide

• Length: 321 bp

>T159306 NZ_CP069996:5098103-5098210 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13415.12 Da
• Isoelectric Point: 5.5286

>AT159306 WP_047715718.1 NZ_CP054278:c3457956-3457591 [Citrobacter freundii]
MQSTTLYGSSAENASCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFSDDEALRLDLAFPLIMEQLERMLTTGE
LDPRSQHCATLHHNGLVCEADTLGSHGYVYIAIYPQSAVTR

Nucleotide

• Length: 366 bp

>AT159306 NZ_CP069996:c5098050-5097989 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT