Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2952987..2953607 Replicon chromosome
Accession NZ_CP054278
Organism Citrobacter freundii strain IDR1900015725-01-02

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HS092_RS14395 Protein ID WP_002892050.1
Coordinates 2953389..2953607 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HS092_RS14390 Protein ID WP_003021733.1
Coordinates 2952987..2953361 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS092_RS14380 2948133..2949326 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HS092_RS14385 2949349..2952498 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HS092_RS14390 2952987..2953361 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HS092_RS14395 2953389..2953607 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HS092_RS14400 2953914..2954465 + 552 WP_032936967.1 maltose O-acetyltransferase -
HS092_RS14405 2954582..2955052 + 471 WP_003021724.1 YlaC family protein -
HS092_RS14410 2955131..2955271 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HS092_RS14415 2955273..2955533 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HS092_RS14420 2955722..2957275 + 1554 WP_003835927.1 EAL domain-containing protein -
HS092_RS14425 2957327..2957680 - 354 WP_003831033.1 DUF1428 family protein -
HS092_RS14430 2957745..2958374 - 630 WP_016149709.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159305 WP_002892050.1 NZ_CP054278:2953389-2953607 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T159305 NZ_CP069996:4823275-4823553 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT159305 WP_003021733.1 NZ_CP054278:2952987-2953361 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT159305 NZ_CP069996:4823553-4823837 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References