Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 133568..134229 Replicon chromosome
Accession NZ_CP054278
Organism Citrobacter freundii strain IDR1900015725-01-02

Toxin (Protein)


Gene name ypjF Uniprot ID S3IMJ6
Locus tag HS092_RS00725 Protein ID WP_000698542.1
Coordinates 133906..134229 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID S3J179
Locus tag HS092_RS00720 Protein ID WP_000065326.1
Coordinates 133568..133885 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS092_RS00675 128708..129532 + 825 WP_000197385.1 DUF945 domain-containing protein -
HS092_RS00680 129741..130451 + 711 WP_024198336.1 hypothetical protein -
HS092_RS00685 130477..131013 + 537 WP_000219797.1 DUF4339 domain-containing protein -
HS092_RS00690 131055..131492 + 438 WP_024198335.1 hypothetical protein -
HS092_RS00695 131559..131969 + 411 WP_000912997.1 hypothetical protein -
HS092_RS00700 132047..132283 + 237 WP_000004273.1 DUF905 domain-containing protein -
HS092_RS00705 132369..132827 + 459 WP_016538084.1 antirestriction protein -
HS092_RS00710 132843..133319 + 477 WP_000811694.1 RadC family protein -
HS092_RS00715 133328..133549 + 222 WP_000691995.1 DUF987 domain-containing protein -
HS092_RS00720 133568..133885 + 318 WP_000065326.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HS092_RS00725 133906..134229 + 324 WP_000698542.1 TA system toxin CbtA family protein Toxin
HS092_RS00730 134470..135006 - 537 WP_049001048.1 GNAT family N-acetyltransferase -
HS092_RS00735 135823..138285 + 2463 WP_032949991.1 poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12350.38 Da        Isoelectric Point: 8.9789

>T159295 WP_000698542.1 NZ_CP054278:133906-134229 [Citrobacter freundii]
MKILPATISRAAKPCLPPVAVWQLLLTRLLEKHYGLTLNDTPFSDETVIKEHFDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTNIDIMRARRDLGLLNRN

Download         Length: 324 bp

>T159295 NZ_CP069996:2477078-2477185 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCACGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11830.50 Da        Isoelectric Point: 6.9367

>AT159295 WP_000065326.1 NZ_CP054278:133568-133885 [Citrobacter freundii]
MSNIIWGLQRDITPRLGTRLVQEGNQLHYLADRACITGKFSDAECLKLDVAFPHFISRMESMLSTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYVAVYSTQR

Download         Length: 318 bp

>AT159295 NZ_CP069996:c2477029-2476964 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S3IMJ6

Antitoxin

Source ID Structure
AlphaFold DB S3J179

References