Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 69215..69951 Replicon plasmid pOSUMEAT1
Accession NZ_CP054271
Organism Raoultella ornithinolytica strain OHMEATKPC2_NDM5

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HRV96_RS26200 Protein ID WP_003026803.1
Coordinates 69469..69951 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HRV96_RS26195 Protein ID WP_003026799.1
Coordinates 69215..69481 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRV96_RS26150 (HRV96_26065) 64554..64988 + 435 WP_234972288.1 copper-binding protein -
HRV96_RS26155 65168..65330 - 163 Protein_79 type II toxin-antitoxin system RelE/ParE family toxin -
HRV96_RS26160 (HRV96_26075) 65452..65631 - 180 WP_004118349.1 type II toxin-antitoxin system ParD family antitoxin -
HRV96_RS26165 (HRV96_26080) 65945..66214 + 270 WP_003026780.1 hypothetical protein -
HRV96_RS26170 (HRV96_26085) 66202..66774 + 573 WP_064360949.1 hypothetical protein -
HRV96_RS26175 (HRV96_26090) 66806..67300 + 495 WP_064360948.1 hypothetical protein -
HRV96_RS26180 (HRV96_26095) 67344..67697 + 354 WP_003026788.1 hypothetical protein -
HRV96_RS26185 (HRV96_26100) 67772..67975 + 204 WP_003026790.1 HHA domain-containing protein -
HRV96_RS26190 (HRV96_26105) 68046..68945 - 900 WP_099000947.1 hypothetical protein -
HRV96_RS26195 (HRV96_26110) 69215..69481 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HRV96_RS26200 (HRV96_26115) 69469..69951 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HRV96_RS26205 (HRV96_26120) 70163..71509 + 1347 WP_020314316.1 ISNCY family transposase -
HRV96_RS27550 71670..71801 + 132 WP_004218042.1 hypothetical protein -
HRV96_RS26210 (HRV96_26125) 72010..72762 - 753 Protein_91 IS3-like element ISKpn38 family transposase -
HRV96_RS26215 (HRV96_26130) 72878..73858 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..180641 180641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T159273 WP_003026803.1 NZ_CP054271:69469-69951 [Raoultella ornithinolytica]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T159273 NZ_CP069993:c169864-169448 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT159273 WP_003026799.1 NZ_CP054271:69215-69481 [Raoultella ornithinolytica]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT159273 NZ_CP069993:c170091-169861 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References