Detailed information of TA system TA159272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 27428..27963
• Replicon: plasmid pOSUMEAT1
• Accession: NZ_CP054271
• Organism: Raoultella ornithinolytica strain OHMEATKPC2_NDM5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: HRV96_RS25985
• Protein ID: WP_032693963.1
• Coordinates: 27676..27963 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8G2A2B7
• Locus tag: HRV96_RS25980
• Protein ID: WP_032752108.1
• Coordinates: 27428..27679 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV96_RS25950 (HRV96_25870)	22794..23073	-	280	Protein_38	hypothetical protein	-
HRV96_RS25955 (HRV96_25875)	23391..23621	-	231	WP_047665741.1	hypothetical protein	-
HRV96_RS25960 (HRV96_25880)	23685..24359	-	675	WP_234972284.1	plasmid partitioning/stability family protein	-
HRV96_RS25965 (HRV96_25885)	24362..25333	-	972	WP_077255421.1	plasmid segregation protein ParM	-
HRV96_RS25970 (HRV96_25890)	25552..25995	+	444	WP_040118420.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HRV96_RS25975 (HRV96_25895)	25995..27263	+	1269	WP_032752101.1	Y-family DNA polymerase	-
HRV96_RS25980 (HRV96_25900)	27428..27679	+	252	WP_032752108.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HRV96_RS25985 (HRV96_25905)	27676..27963	+	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRV96_RS25990 (HRV96_25910)	29056..29328	+	273	Protein_46	DNA polymerase V subunit UmuC	-
HRV96_RS25995 (HRV96_25915)	30129..31100	-	972	WP_032752114.1	ParB/RepB/Spo0J family plasmid partition protein	-
HRV96_RS26000 (HRV96_25920)	31100..32266	-	1167	WP_032752115.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..180641	180641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T159272 WP_032693963.1 NZ_CP054271:27676-27963 [Raoultella ornithinolytica]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T159272 NZ_CP069993:c124929-124447 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9174.43 Da
• Isoelectric Point: 3.9507

>AT159272 WP_032752108.1 NZ_CP054271:27428-27679 [Raoultella ornithinolytica]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQQLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT159272 NZ_CP069993:c125183-124917 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2