Detailed information of TA system TA159271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4677766..4678345
• Replicon: chromosome
• Accession: NZ_CP054270
• Organism: Raoultella ornithinolytica strain OHMEATKPC2_NDM5

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: HRV96_RS22175
• Protein ID: WP_032716505.1
• Coordinates: 4678058..4678345 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HRV96_RS22170
• Protein ID: WP_032716507.1
• Coordinates: 4677766..4678071 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV96_RS22145 (HRV96_22080)	4672974..4674038	-	1065	WP_004857527.1	DUF2955 domain-containing protein	-
HRV96_RS22150 (HRV96_22085)	4674028..4675095	-	1068	WP_004857525.1	HlyD family secretion protein	-
HRV96_RS22155 (HRV96_22090)	4675102..4675566	-	465	WP_004857523.1	MarR family transcriptional regulator	-
HRV96_RS22160 (HRV96_22095)	4675857..4676021	-	165	WP_004857521.1	DUF1127 domain-containing protein	-
HRV96_RS22165 (HRV96_22100)	4676199..4677611	+	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
HRV96_RS22170 (HRV96_22105)	4677766..4678071	-	306	WP_032716507.1	BrnA antitoxin family protein	Antitoxin
HRV96_RS22175 (HRV96_22110)	4678058..4678345	-	288	WP_032716505.1	BrnT family toxin	Toxin
HRV96_RS22180 (HRV96_22115)	4678462..4678881	-	420	WP_004857515.1	FosA family fosfomycin resistance glutathione transferase	-
HRV96_RS22185 (HRV96_22120)	4678875..4679783	-	909	WP_004857513.1	LysR family transcriptional regulator	-
HRV96_RS22190 (HRV96_22125)	4679871..4680653	+	783	WP_032716502.1	NAD(P)H-dependent oxidoreductase	-
HRV96_RS22195 (HRV96_22130)	4680801..4681379	+	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
HRV96_RS22200 (HRV96_22135)	4681523..4682335	+	813	WP_104896991.1	helix-turn-helix domain-containing protein	-
HRV96_RS22205 (HRV96_22140)	4682332..4682850	+	519	WP_015585229.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T159271 WP_032716505.1 NZ_CP054270:c4678345-4678058 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T159271 NZ_CP069992:4911872-4912405 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTAGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11711.24 Da
• Isoelectric Point: 9.4429

>AT159271 WP_032716507.1 NZ_CP054270:c4678071-4677766 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALTKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKKS

Nucleotide

• Length: 306 bp

>AT159271 NZ_CP069992:4911595-4911861 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A808YBH6