Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4061299..4061918 Replicon chromosome
Accession NZ_CP054270
Organism Raoultella ornithinolytica strain OHMEATKPC2_NDM5

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag HRV96_RS19235 Protein ID WP_004858783.1
Coordinates 4061700..4061918 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag HRV96_RS19230 Protein ID WP_004858785.1
Coordinates 4061299..4061673 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRV96_RS19220 (HRV96_19160) 4056409..4057602 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HRV96_RS19225 (HRV96_19165) 4057625..4060771 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit AcrB -
HRV96_RS19230 (HRV96_19170) 4061299..4061673 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
HRV96_RS19235 (HRV96_19175) 4061700..4061918 + 219 WP_004858783.1 HHA domain-containing protein Toxin
HRV96_RS19240 (HRV96_19180) 4062070..4062636 + 567 WP_004858781.1 maltose O-acetyltransferase -
HRV96_RS19245 4062608..4062742 - 135 WP_223306663.1 hypothetical protein -
HRV96_RS19250 (HRV96_19185) 4062736..4063206 + 471 WP_128318549.1 YlaC family protein -
HRV96_RS19255 (HRV96_19190) 4063181..4064632 - 1452 WP_032716766.1 PLP-dependent aminotransferase family protein -
HRV96_RS19260 (HRV96_19195) 4064736..4065446 + 711 WP_015585015.1 GNAT family protein -
HRV96_RS19265 (HRV96_19200) 4065443..4065583 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HRV96_RS19270 (HRV96_19205) 4065586..4065846 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T159270 WP_004858783.1 NZ_CP054270:4061700-4061918 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T159270 NZ_CP069992:4797532-4797816 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTTAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT159270 WP_004858785.1 NZ_CP054270:4061299-4061673 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT159270 NZ_CP069992:4797300-4797542 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References