Detailed information of TA system TA159259

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 789467..790124
• Replicon: chromosome
• Accession: NZ_CP054270
• Organism: Raoultella ornithinolytica strain OHMEATKPC2_NDM5

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HRV96_RS03850
• Protein ID: WP_088912076.1
• Coordinates: 789714..790124 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A514ELX1
• Locus tag: HRV96_RS03845
• Protein ID: WP_004867358.1
• Coordinates: 789467..789733 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV96_RS03820 (HRV96_03815)	784601..786034	-	1434	WP_071359760.1	6-phospho-beta-glucosidase	-
HRV96_RS03825 (HRV96_03820)	786154..786882	-	729	WP_004867368.1	MurR/RpiR family transcriptional regulator	-
HRV96_RS03830 (HRV96_03825)	786932..787243	+	312	WP_004867365.1	N(4)-acetylcytidine aminohydrolase	-
HRV96_RS03835 (HRV96_03830)	787405..788064	+	660	WP_004867362.1	hemolysin III family protein	-
HRV96_RS03840 (HRV96_03835)	788187..789170	-	984	WP_015585783.1	tRNA-modifying protein YgfZ	-
HRV96_RS03845 (HRV96_03840)	789467..789733	+	267	WP_004867358.1	FAD assembly factor SdhE	Antitoxin
HRV96_RS03850 (HRV96_03845)	789714..790124	+	411	WP_088912076.1	protein YgfX	Toxin
HRV96_RS03855 (HRV96_03850)	790132..790653	-	522	WP_004867353.1	flavodoxin FldB	-
HRV96_RS03860 (HRV96_03855)	790831..791727	+	897	WP_032718225.1	site-specific tyrosine recombinase XerD	-
HRV96_RS03865 (HRV96_03860)	791750..792463	+	714	WP_004867349.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HRV96_RS03870 (HRV96_03865)	792469..794202	+	1734	WP_015585785.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16244.19 Da
• Isoelectric Point: 11.3349

>T159259 WP_088912076.1 NZ_CP054270:789714-790124 [Raoultella ornithinolytica]
VVLWQSDLRISWRSQWFSLLLHGIVAAIVLLMPWPLSYTPLWLLLLSMVVFDSVRSQRRIHACRGEIKLMTDSRLRWQKA
EWEIVGTPWVINSGMLLRLQDMQTRRRQHLWIAADSMDAKEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T159259 NZ_CP069991:124332-124619 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10553.05 Da
• Isoelectric Point: 4.8432

>AT159259 WP_004867358.1 NZ_CP054270:789467-789733 [Raoultella ornithinolytica]
MDITNKARIHWACRRGMRELDISIMPFFEYEYDTLSDEDKQLFIRLLENDDPDLFNWLMNHGEPADAQLQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT159259 NZ_CP069991:124084-124335 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A514ELX1