Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4761..5308 Replicon chromosome
Accession NZ_CP054270
Organism Raoultella ornithinolytica strain OHMEATKPC2_NDM5

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag HRV96_RS00035 Protein ID WP_048025274.1
Coordinates 4761..5069 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag HRV96_RS00040 Protein ID WP_004869059.1
Coordinates 5072..5308 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRV96_RS00010 (HRV96_00010) 377..1702 - 1326 WP_004869064.1 PTS cellobiose transporter subunit IIC -
HRV96_RS00015 (HRV96_00015) 1714..2028 - 315 WP_004869063.1 PTS sugar transporter subunit IIB -
HRV96_RS00020 (HRV96_00020) 2323..3264 + 942 WP_015585487.1 LacI family DNA-binding transcriptional regulator -
HRV96_RS00025 (HRV96_00025) 3359..3634 - 276 WP_162769256.1 YicS family protein -
HRV96_RS00030 (HRV96_00030) 3743..4645 - 903 WP_015585496.1 EamA family transporter -
HRV96_RS00035 (HRV96_00035) 4761..5069 - 309 WP_048025274.1 CcdB family protein Toxin
HRV96_RS00040 (HRV96_00040) 5072..5308 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HRV96_RS00045 (HRV96_00045) 5414..6847 - 1434 WP_069476354.1 PTS N-acetylmuramic acid transporter subunit IIBC -
HRV96_RS00050 (HRV96_00050) 6872..7774 - 903 WP_088402359.1 N-acetylmuramic acid 6-phosphate etherase -
HRV96_RS00055 (HRV96_00055) 7939..9102 - 1164 WP_004869055.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T159258 WP_048025274.1 NZ_CP054270:c5069-4761 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T159258 NZ_CP069991:c64678-64529 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT159258 WP_004869059.1 NZ_CP054270:c5308-5072 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT159258 NZ_CP069991:64726-64782 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References