Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5336233..5336858 Replicon chromosome
Accession NZ_CP054268
Organism Klebsiella pneumoniae strain 39427

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HPS02_RS28245 Protein ID WP_002882817.1
Coordinates 5336233..5336616 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HPS02_RS28250 Protein ID WP_004150355.1
Coordinates 5336616..5336858 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPS02_RS28230 5333599..5334501 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HPS02_RS28235 5334498..5335133 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HPS02_RS28240 5335130..5336059 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HPS02_RS28245 5336233..5336616 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HPS02_RS28250 5336616..5336858 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HPS02_RS28255 5337063..5337980 + 918 WP_002882812.1 alpha/beta hydrolase -
HPS02_RS28260 5337994..5338935 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HPS02_RS28265 5338980..5339417 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HPS02_RS28270 5339414..5340274 - 861 WP_002882807.1 virulence factor BrkB family protein -
HPS02_RS28275 5340268..5340867 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T159257 WP_002882817.1 NZ_CP054268:c5336616-5336233 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T159257 NZ_CP069990:4909138-4909473 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT159257 WP_004150355.1 NZ_CP054268:c5336858-5336616 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT159257 NZ_CP069990:4908890-4909138 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References