Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4756200..4757010 Replicon chromosome
Accession NZ_CP054268
Organism Klebsiella pneumoniae strain 39427

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HPS02_RS25475 Protein ID WP_002887280.1
Coordinates 4756200..4756733 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HPS02_RS25480 Protein ID WP_002887278.1
Coordinates 4756744..4757010 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPS02_RS25470 4755031..4756152 + 1122 WP_002887282.1 cupin domain-containing protein -
HPS02_RS25475 4756200..4756733 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
HPS02_RS25480 4756744..4757010 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HPS02_RS25485 4757113..4758546 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
HPS02_RS25490 4758536..4759219 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HPS02_RS25495 4759391..4760776 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
HPS02_RS25500 4760794..4761138 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T159254 WP_002887280.1 NZ_CP054268:c4756733-4756200 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T159254 NZ_CP069990:3992649-3992756 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT159254 WP_002887278.1 NZ_CP054268:c4757010-4756744 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT159254 NZ_CP069990:c3992592-3992535 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References