Detailed information of TA system TA159235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 2846733..2847286
• Replicon: chromosome
• Accession: NZ_CP054264
• Organism: Pseudomonas putida strain A0_F0_I1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q88JZ3
• Locus tag: HG554_RS13095
• Protein ID: WP_010953434.1
• Coordinates: 2846993..2847286 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q88JZ4
• Locus tag: HG554_RS13090
• Protein ID: WP_010953433.1
• Coordinates: 2846733..2847005 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG554_RS13060 (HG554_13065)	2842305..2842664	-	360	Protein_2544	DNA repair protein RadC	-
HG554_RS13065 (HG554_13070)	2842636..2843586	-	951	WP_031299400.1	hypothetical protein	-
HG554_RS13070 (HG554_13075)	2843679..2844683	-	1005	WP_010953429.1	YqaJ viral recombinase family protein	-
HG554_RS13075 (HG554_13080)	2844763..2845731	-	969	WP_031299399.1	DUF932 domain-containing protein	-
HG554_RS13080 (HG554_13085)	2845829..2846158	-	330	WP_010953431.1	hypothetical protein	-
HG554_RS13085 (HG554_13090)	2846363..2846608	+	246	WP_010953432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HG554_RS13090 (HG554_13095)	2846733..2847005	+	273	WP_010953433.1	CopG family ribbon-helix-helix protein	Antitoxin
HG554_RS13095 (HG554_13100)	2846993..2847286	+	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HG554_RS13100 (HG554_13105)	2847280..2848689	-	1410	WP_010953435.1	site-specific integrase	-
HG554_RS13105 (HG554_13110)	2849212..2850138	-	927	WP_010953436.1	LysR family transcriptional regulator	-
HG554_RS13110 (HG554_13115)	2850252..2850983	+	732	WP_010953437.1	SDR family NAD(P)-dependent oxidoreductase	-
HG554_RS13115 (HG554_13120)	2851053..2851262	+	210	WP_010953438.1	4-oxalocrotonate tautomerase family protein	-
HG554_RS13120 (HG554_13125)	2851259..2851471	+	213	WP_061405625.1	hypothetical protein	-
HG554_RS13125 (HG554_13130)	2851614..2852132	+	519	WP_049587643.1	DUF1993 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2816293..2860265	43972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11155.61 Da
• Isoelectric Point: 5.9081

>T159235 WP_010953434.1 NZ_CP054264:2846993-2847286 [Pseudomonas putida]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T159235 NZ_CP069990:659019-659270 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10373.53 Da
• Isoelectric Point: 5.5744

>AT159235 WP_010953433.1 NZ_CP054264:2846733-2847005 [Pseudomonas putida]
MATSIKIDDDLKGRIQHLACLRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGQHLTGQEARTWLGSWGTD
AEAELPKCHD

Nucleotide

• Length: 273 bp

>AT159235 NZ_CP069990:659267-659521 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A2W5CNE8

Antitoxin

• AlphaFold DB: Q88JZ4