Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 2780300..2781183 Replicon chromosome
Accession NZ_CP054264
Organism Pseudomonas putida strain A0_F0_I1

Toxin (Protein)


Gene name - Uniprot ID Q88K57
Locus tag HG554_RS12715 Protein ID WP_003250527.1
Coordinates 2780746..2781183 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID I7C3Q7
Locus tag HG554_RS12710 Protein ID WP_003250525.1
Coordinates 2780300..2780749 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG554_RS12685 (HG554_12690) 2775724..2776923 + 1200 WP_049587621.1 sugar transporter -
HG554_RS12690 (HG554_12695) 2776963..2777547 - 585 WP_010953382.1 LysE family transporter -
HG554_RS12695 (HG554_12700) 2777598..2778428 - 831 WP_003250518.1 AraC family transcriptional regulator -
HG554_RS12700 (HG554_12705) 2778477..2779400 - 924 WP_010953383.1 LysR family transcriptional regulator -
HG554_RS12705 (HG554_12710) 2779504..2780157 + 654 WP_010953384.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
HG554_RS12710 (HG554_12715) 2780300..2780749 + 450 WP_003250525.1 DUF2384 domain-containing protein Antitoxin
HG554_RS12715 (HG554_12720) 2780746..2781183 + 438 WP_003250527.1 RES family NAD+ phosphorylase Toxin
HG554_RS12720 (HG554_12725) 2781191..2782345 - 1155 WP_049587669.1 aminotransferase class V-fold PLP-dependent enzyme -
HG554_RS12725 (HG554_12730) 2782539..2783465 - 927 WP_010953387.1 LysR family transcriptional regulator -
HG554_RS12730 (HG554_12735) 2783606..2784835 + 1230 WP_049587623.1 acyl-CoA dehydrogenase -
HG554_RS12735 (HG554_12740) 2784999..2785247 + 249 Protein_2482 PAAR domain-containing protein -
HG554_RS12740 (HG554_12745) 2785250..2785639 + 390 WP_049587628.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15990.29 Da        Isoelectric Point: 5.6007

>T159234 WP_003250527.1 NZ_CP054264:2780746-2781183 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Download         Length: 438 bp

>T159234 NZ_CP069982:81700-81834 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16956.46 Da        Isoelectric Point: 5.7258

>AT159234 WP_003250525.1 NZ_CP054264:2780300-2780749 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Download         Length: 450 bp

>AT159234 NZ_CP069982:c81655-81595 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GW6

Antitoxin

Source ID Structure
AlphaFold DB I7C3Q7

References