Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-higA/HigB-HigA
Location 332741..333732 Replicon chromosome
Accession NZ_CP054264
Organism Pseudomonas putida strain A0_F0_I1

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag HG554_RS01435 Protein ID WP_020190010.1
Coordinates 332741..333217 (+) Length 159 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q88R60
Locus tag HG554_RS01440 Protein ID WP_004575922.1
Coordinates 333259..333732 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG554_RS01415 (HG554_01415) 327884..329251 - 1368 WP_010951639.1 ATP-binding protein -
HG554_RS01420 (HG554_01420) 329253..329972 - 720 WP_010951640.1 response regulator -
HG554_RS01425 (HG554_01425) 330113..332410 + 2298 WP_010951641.1 TonB-dependent siderophore receptor -
HG554_RS01430 (HG554_01430) 332476..332682 - 207 WP_014860378.1 DUF3079 domain-containing protein -
HG554_RS01435 (HG554_01435) 332741..333217 + 477 WP_020190010.1 type II toxin-antitoxin system HigB family toxin Toxin
HG554_RS01440 (HG554_01440) 333259..333732 + 474 WP_004575922.1 transcriptional regulator Antitoxin
HG554_RS01445 (HG554_01445) 333737..333924 - 188 Protein_282 integrase -
HG554_RS01450 (HG554_01450) 334152..334367 + 216 WP_010951644.1 type II toxin-antitoxin system RelE/ParE family toxin -
HG554_RS01455 (HG554_01455) 334364..334687 - 324 WP_010951645.1 helix-turn-helix transcriptional regulator -
HG554_RS01465 (HG554_01465) 335149..335397 - 249 WP_010951646.1 hypothetical protein -
HG554_RS01470 (HG554_01470) 335918..336193 + 276 WP_049588069.1 DUF3077 domain-containing protein -
HG554_RS01475 (HG554_01475) 336548..337753 - 1206 WP_010951648.1 methyltransferase -
HG554_RS01480 (HG554_01480) 337882..338571 - 690 WP_010951649.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(114-152)

Antitoxin

Antitoxin HigA

(43-154)


Sequences

Toxin        


Download         Length: 159 a.a.        Molecular weight: 18386.38 Da        Isoelectric Point: 10.3522

>T159230 WP_020190010.1 NZ_CP054264:332741-333217 [Pseudomonas putida]
MTGINPLAIARSPYIAHPDIWLTDIWIGDIFHPMLVRRDNTMRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFES
FEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIKKNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Download         Length: 477 bp

>T159230 NZ_CP069981:c22881-22630 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17229.67 Da        Isoelectric Point: 4.4982

>AT159230 WP_004575922.1 NZ_CP054264:333259-333732 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Download         Length: 474 bp

>AT159230 NZ_CP069981:c22643-22416 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CBJ4

References