Detailed information of TA system TA159230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 332741..333732
• Replicon: chromosome
• Accession: NZ_CP054264
• Organism: Pseudomonas putida strain A0_F0_I1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HG554_RS01435
• Protein ID: WP_020190010.1
• Coordinates: 332741..333217 (+)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88R60
• Locus tag: HG554_RS01440
• Protein ID: WP_004575922.1
• Coordinates: 333259..333732 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG554_RS01415 (HG554_01415)	327884..329251	-	1368	WP_010951639.1	ATP-binding protein	-
HG554_RS01420 (HG554_01420)	329253..329972	-	720	WP_010951640.1	response regulator	-
HG554_RS01425 (HG554_01425)	330113..332410	+	2298	WP_010951641.1	TonB-dependent siderophore receptor	-
HG554_RS01430 (HG554_01430)	332476..332682	-	207	WP_014860378.1	DUF3079 domain-containing protein	-
HG554_RS01435 (HG554_01435)	332741..333217	+	477	WP_020190010.1	type II toxin-antitoxin system HigB family toxin	Toxin
HG554_RS01440 (HG554_01440)	333259..333732	+	474	WP_004575922.1	transcriptional regulator	Antitoxin
HG554_RS01445 (HG554_01445)	333737..333924	-	188	Protein_282	integrase	-
HG554_RS01450 (HG554_01450)	334152..334367	+	216	WP_010951644.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HG554_RS01455 (HG554_01455)	334364..334687	-	324	WP_010951645.1	helix-turn-helix transcriptional regulator	-
HG554_RS01465 (HG554_01465)	335149..335397	-	249	WP_010951646.1	hypothetical protein	-
HG554_RS01470 (HG554_01470)	335918..336193	+	276	WP_049588069.1	DUF3077 domain-containing protein	-
HG554_RS01475 (HG554_01475)	336548..337753	-	1206	WP_010951648.1	methyltransferase	-
HG554_RS01480 (HG554_01480)	337882..338571	-	690	WP_010951649.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (114-152)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 18386.38 Da
• Isoelectric Point: 10.3522

>T159230 WP_020190010.1 NZ_CP054264:332741-333217 [Pseudomonas putida]
MTGINPLAIARSPYIAHPDIWLTDIWIGDIFHPMLVRRDNTMRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFES
FEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIKKNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 477 bp

>T159230 NZ_CP069981:c22881-22630 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17229.67 Da
• Isoelectric Point: 4.4982

>AT159230 WP_004575922.1 NZ_CP054264:333259-333732 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT159230 NZ_CP069981:c22643-22416 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CBJ4