Detailed information of TA system TA159218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 127189..127756
• Replicon: chromosome
• Accession: NZ_CP054257
• Organism: Treponema parvum strain ATCC 700773

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: HRI96_RS00620
• Protein ID: WP_210117624.1
• Coordinates: 127189..127476 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HRI96_RS00625
• Protein ID: WP_256729258.1
• Coordinates: 127463..127756 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRI96_RS00580 (HRI96_00585)	122599..123747	-	1149	WP_210117617.1	radical SAM protein	-
HRI96_RS00585 (HRI96_00590)	123808..124056	-	249	WP_210117618.1	ACGX-repeat peptide	-
HRI96_RS00590 (HRI96_00595)	124142..124474	-	333	WP_210117619.1	helix-turn-helix domain-containing protein	-
HRI96_RS00595 (HRI96_00600)	124565..125257	-	693	WP_256729257.1	4Fe-4S binding protein	-
HRI96_RS00600 (HRI96_00605)	125517..125744	+	228	WP_210117620.1	DUF2281 domain-containing protein	-
HRI96_RS00605 (HRI96_00610)	125741..126118	+	378	WP_210117621.1	type II toxin-antitoxin system VapC family toxin	-
HRI96_RS00610 (HRI96_00615)	126386..126784	-	399	WP_210117622.1	type II toxin-antitoxin system VapC family toxin	-
HRI96_RS00615 (HRI96_00620)	126784..127005	-	222	WP_210117623.1	hypothetical protein	-
HRI96_RS00620 (HRI96_00625)	127189..127476	+	288	WP_210117624.1	BrnT family toxin	Toxin
HRI96_RS00625 (HRI96_00630)	127463..127756	+	294	WP_256729258.1	BrnA antitoxin family protein	Antitoxin
HRI96_RS00630 (HRI96_00635)	127821..128117	-	297	WP_210117626.1	HigA family addiction module antitoxin	-
HRI96_RS00635 (HRI96_00640)	128127..128282	-	156	Protein_128	type II toxin-antitoxin system RelE/ParE family toxin	-
HRI96_RS00640 (HRI96_00645)	128425..129078	+	654	WP_210117627.1	response regulator transcription factor	-
HRI96_RS00645 (HRI96_00650)	129131..129715	+	585	WP_256729259.1	hypothetical protein	-
HRI96_RS00650 (HRI96_00655)	129712..130596	+	885	WP_210117629.1	histidine kinase	-
HRI96_RS00655 (HRI96_00660)	130896..131624	+	729	WP_210117630.1	leucine-rich repeat domain-containing protein	-
HRI96_RS00660 (HRI96_00665)	131812..132750	+	939	WP_210117631.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	19890..134522	114632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (29-95)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11362.65 Da
• Isoelectric Point: 4.5527

>T159218 WP_210117624.1 NZ_CP054257:127189-127476 [Treponema parvum]
MTFEWDENINRINKEIHDGISFEYAARVFLDSKRIEIVDENHSDESEEGCNIIGCVERILFAVYTERNDNKIRIISARRA
TPKEEKLYYENYDIR

Nucleotide

• Length: 288 bp

>T159218 NZ_CP069980:c2682995-2682654 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11043.83 Da
• Isoelectric Point: 6.9790

>AT159218 WP_256729258.1 NZ_CP054257:127463-127756 [Treponema parvum]
MTLDDLIKNLLTEEDRKIINSADPVITEECPEVTDAQMKKYKPWYEVHPKGNGIYKVSVKKTAVSLRIDTDVLMALKEMG
TGYQTRINDILRKAVFG

Nucleotide

• Length: 294 bp

>AT159218 NZ_CP069980:2682990-2683066 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT