Detailed information of TA system TA159216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 80227..80966
• Replicon: plasmid unnamed
• Accession: NZ_CP054255
• Organism: Klebsiella variicola strain FH-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HL854_RS27160
• Protein ID: WP_065907730.1
• Coordinates: 80227..80712 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: HL854_RS27165
• Protein ID: WP_012540086.1
• Coordinates: 80700..80966 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL854_RS27130	75676..76206	-	531	WP_065907732.1	antirestriction protein	-
HL854_RS27135	76831..77664	-	834	WP_040107920.1	type I restriction-modification system subunit M	-
HL854_RS27140	77711..77941	-	231	WP_038990977.1	hypothetical protein	-
HL854_RS27145	78029..78376	-	348	WP_038990975.1	hypothetical protein	-
HL854_RS27150	78443..78823	-	381	WP_038990973.1	hypothetical protein	-
HL854_RS27155	79727..79885	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
HL854_RS27160	80227..80712	-	486	WP_065907730.1	GNAT family N-acetyltransferase	Toxin
HL854_RS27165	80700..80966	-	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
HL854_RS27170	81266..81589	+	324	WP_065907728.1	metalloregulator ArsR/SmtB family transcription factor	-
HL854_RS27175	81591..82304	+	714	WP_013087170.1	arsenical resistance protein ArsH	-
HL854_RS27180	82313..82858	+	546	WP_047365773.1	sigma-70 family RNA polymerase sigma factor	-
HL854_RS27185	82934..83296	+	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
HL854_RS27190	83323..85074	+	1752	WP_162875198.1	arsenical pump-driving ATPase	-
HL854_RS27195	85199..85735	+	537	WP_013087166.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..110148	110148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17840.65 Da
• Isoelectric Point: 9.8560

>T159216 WP_065907730.1 NZ_CP054255:c80712-80227 [Klebsiella variicola]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKSLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T159216 NZ_CP069980:2414139-2414312 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT159216 WP_012540086.1 NZ_CP054255:c80966-80700 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT159216 NZ_CP069980:2413815-2414111 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A5E1AWX8