Detailed information of TA system TA159215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 13684..14327
• Replicon: plasmid unnamed
• Accession: NZ_CP054255
• Organism: Klebsiella variicola strain FH-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HL854_RS26800
• Protein ID: WP_173818490.1
• Coordinates: 13911..14327 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HL854_RS26795
• Protein ID: WP_049010641.1
• Coordinates: 13684..13914 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL854_RS26775	9330..9587	-	258	WP_023292103.1	hypothetical protein	-
HL854_RS26780	10190..11644	+	1455	WP_074180203.1	EAL domain-containing protein	-
HL854_RS26785	12178..12708	-	531	Protein_10	hypothetical protein	-
HL854_RS26790	12759..13109	-	351	WP_023292071.1	hypothetical protein	-
HL854_RS26795	13684..13914	+	231	WP_049010641.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HL854_RS26800	13911..14327	+	417	WP_173818490.1	type II toxin-antitoxin system VapC family toxin	Toxin
HL854_RS26805	14378..18172	-	3795	WP_173818491.1	pcar	-
HL854_RS26810	18445..18708	+	264	WP_009310051.1	hypothetical protein	-
HL854_RS26815	18705..19271	+	567	WP_173818493.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..110148	110148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15074.57 Da
• Isoelectric Point: 7.8874

>T159215 WP_173818490.1 NZ_CP054255:13911-14327 [Klebsiella variicola]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVLLVDAFCARVDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLILEDWVK

Nucleotide

• Length: 417 bp

>T159215 NZ_CP069980:c1789800-1789615 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8681.76 Da
• Isoelectric Point: 4.4692

>AT159215 WP_049010641.1 NZ_CP054255:13684-13914 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFASLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT159215 NZ_CP069980:c1789598-1789188 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA