Detailed information of TA system TA159214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5454516..5455141
• Replicon: chromosome
• Accession: NZ_CP054254
• Organism: Klebsiella variicola strain FH-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1F2M041
• Locus tag: HL854_RS26325
• Protein ID: WP_008807903.1
• Coordinates: 5454516..5454899 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HL854_RS26330
• Protein ID: WP_162550505.1
• Coordinates: 5454899..5455141 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL854_RS26310	5451882..5452784	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HL854_RS26315	5452781..5453416	+	636	WP_008807902.1	formate dehydrogenase cytochrome b556 subunit	-
HL854_RS26320	5453413..5454342	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
HL854_RS26325	5454516..5454899	-	384	WP_008807903.1	type II toxin-antitoxin system VapC family toxin	Toxin
HL854_RS26330	5454899..5455141	-	243	WP_162550505.1	ribbon-helix-helix domain-containing protein	Antitoxin
HL854_RS26335	5455345..5456262	+	918	WP_060876598.1	alpha/beta hydrolase	-
HL854_RS26340	5456277..5457218	-	942	WP_012543287.1	fatty acid biosynthesis protein FabY	-
HL854_RS26345	5457263..5457700	-	438	WP_012543288.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HL854_RS26350	5457697..5458557	-	861	WP_008807907.1	virulence factor BrkB family protein	-
HL854_RS26355	5458551..5459150	-	600	WP_008807908.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T159214 WP_008807903.1 NZ_CP054254:c5454899-5454516 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Nucleotide

• Length: 384 bp

>T159214 NZ_CP069980:1304044-1304508 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGGCAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9524.80 Da
• Isoelectric Point: 4.2500

>AT159214 WP_162550505.1 NZ_CP054254:c5455141-5454899 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDLAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT159214 NZ_CP069980:1303709-1304044 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A1F2M041