Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4238099..4238718 Replicon chromosome
Accession NZ_CP054254
Organism Klebsiella variicola strain FH-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HL854_RS20585 Protein ID WP_002892050.1
Coordinates 4238500..4238718 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag HL854_RS20580 Protein ID WP_008805436.1
Coordinates 4238099..4238473 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HL854_RS20570 4233254..4234447 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HL854_RS20575 4234470..4237616 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
HL854_RS20580 4238099..4238473 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
HL854_RS20585 4238500..4238718 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HL854_RS20590 4238877..4239443 + 567 WP_008805435.1 maltose O-acetyltransferase -
HL854_RS20595 4239415..4239543 - 129 Protein_4044 hypothetical protein -
HL854_RS20600 4239580..4240050 + 471 WP_008805434.1 YlaC family protein -
HL854_RS20605 4240019..4241476 - 1458 WP_022066287.1 PLP-dependent aminotransferase family protein -
HL854_RS20610 4241577..4242275 + 699 WP_012968815.1 GNAT family N-acetyltransferase -
HL854_RS20615 4242272..4242412 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HL854_RS20620 4242412..4242675 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159212 WP_002892050.1 NZ_CP054254:4238500-4238718 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T159212 NZ_CP069980:c1195608-1195312 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT159212 WP_008805436.1 NZ_CP054254:4238099-4238473 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT159212 NZ_CP069980:c1195310-1194915 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References