Detailed information of TA system TA159210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2724882..2726470
• Replicon: chromosome
• Accession: NZ_CP054254
• Organism: Klebsiella variicola strain FH-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1F2MCM0
• Locus tag: HL854_RS13255
• Protein ID: WP_008804919.1
• Coordinates: 2724882..2726204 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2V3KHJ7
• Locus tag: HL854_RS13260
• Protein ID: WP_038422544.1
• Coordinates: 2726204..2726470 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL854_RS13240	2719947..2722448	+	2502	WP_173818172.1	fimbrial biogenesis outer membrane usher protein	-
HL854_RS13245	2722436..2723440	+	1005	WP_173818174.1	type 1 fimbrial protein	-
HL854_RS13250	2723573..2724865	+	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
HL854_RS13255	2724882..2726204	-	1323	WP_008804919.1	type II toxin-antitoxin system HipA family toxin	Toxin
HL854_RS13260	2726204..2726470	-	267	WP_038422544.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HL854_RS13265	2726857..2727183	-	327	WP_008804921.1	hydroxyisourate hydrolase	-
HL854_RS13270	2727180..2727680	-	501	WP_008804922.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HL854_RS13275	2727658..2729037	-	1380	WP_012968246.1	purine permease	-
HL854_RS13280	2729383..2730537	+	1155	WP_008804924.1	FAD-dependent urate hydroxylase HpxO	-
HL854_RS13285	2730518..2731441	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48911.26 Da
• Isoelectric Point: 7.9109

>T159210 WP_008804919.1 NZ_CP054254:c2726204-2724882 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T159210 NZ_CP069980:c1046218-1045811 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9923.30 Da
• Isoelectric Point: 8.5912

>AT159210 WP_038422544.1 NZ_CP054254:c2726470-2726204 [Klebsiella variicola]
MTSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT159210 NZ_CP069980:c1046465-1046199 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1F2MCM0

Antitoxin

• AlphaFold DB: A0A2V3KHJ7