Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1820337..1820927 Replicon chromosome
Accession NZ_CP054254
Organism Klebsiella variicola strain FH-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag HL854_RS08760 Protein ID WP_008804165.1
Coordinates 1820595..1820927 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag HL854_RS08755 Protein ID WP_012541132.1
Coordinates 1820337..1820594 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HL854_RS08735 1815944..1816519 + 576 WP_012541129.1 hypothetical protein -
HL854_RS08740 1816698..1817534 + 837 WP_008804155.1 alpha/beta hydrolase -
HL854_RS08745 1817741..1818712 + 972 WP_173818105.1 sensor domain-containing diguanylate cyclase -
HL854_RS08750 1818709..1819809 - 1101 WP_173818107.1 phosphotransferase -
HL854_RS08755 1820337..1820594 + 258 WP_012541132.1 antitoxin Antitoxin
HL854_RS08760 1820595..1820927 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HL854_RS08770 1821250..1822686 + 1437 WP_032740049.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HL854_RS08780 1823059..1824513 - 1455 WP_032740048.1 AMP nucleosidase -
HL854_RS08785 1824644..1824889 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T159201 WP_008804165.1 NZ_CP054254:1820595-1820927 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T159201 NZ_CP069978:c3661059-3660748 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT159201 WP_012541132.1 NZ_CP054254:1820337-1820594 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT159201 NZ_CP069978:c3660747-3660457 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References