Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 327046..327632 Replicon chromosome
Accession NZ_CP054254
Organism Klebsiella variicola strain FH-1

Toxin (Protein)


Gene name doc Uniprot ID A0A2N4YVJ0
Locus tag HL854_RS01495 Protein ID WP_032733892.1
Coordinates 327264..327632 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HL854_RS01490 Protein ID WP_004174006.1
Coordinates 327046..327267 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HL854_RS01470 323204..324130 + 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HL854_RS01475 324127..325404 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
HL854_RS01480 325401..326168 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HL854_RS01485 326170..326883 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HL854_RS01490 327046..327267 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HL854_RS01495 327264..327632 + 369 WP_032733892.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HL854_RS01500 327924..329240 + 1317 WP_008806974.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HL854_RS01505 329347..330234 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HL854_RS01510 330231..331076 + 846 WP_032740290.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HL854_RS01515 331078..332148 + 1071 WP_008806977.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 324127..332885 8758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(8-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13610.96 Da        Isoelectric Point: 7.3191

>T159198 WP_032733892.1 NZ_CP054254:327264-327632 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T159198 NZ_CP069978:2939232-2939384 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT159198 WP_004174006.1 NZ_CP054254:327046-327267 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT159198 NZ_CP069978:c2939183-2939126 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4YVJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References