Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4623219..4623716 Replicon chromosome
Accession NZ_CP054236
Organism Escherichia coli strain EcPF5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HR070_RS22125 Protein ID WP_001173343.1
Coordinates 4623219..4623392 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HR070_RS22130 Protein ID WP_001312331.1
Coordinates 4623420..4623716 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR070_RS22115 4619889..4621346 + 1458 WP_021517943.1 dipeptide/tripeptide permease DtpC -
HR070_RS22120 4621583..4623100 + 1518 WP_000003806.1 lysine--tRNA ligase -
HR070_RS22125 4623219..4623392 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HR070_RS22130 4623420..4623716 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HR070_RS22135 4623945..4624217 - 273 WP_000405647.1 N-acetyltransferase -
HR070_RS22140 4624229..4624459 - 231 WP_000371715.1 (4Fe-4S)-binding protein -
HR070_RS22145 4624640..4626271 + 1632 WP_001216466.1 sensor histidine kinase -
HR070_RS22150 4626268..4626987 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T159184 WP_001173343.1 NZ_CP054236:c4623392-4623219 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T159184 NZ_CP069974:c61942-61808 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT159184 WP_001312331.1 NZ_CP054236:c4623716-4623420 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT159184 NZ_CP069974:61987-62047 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References