Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4448578..4449232 Replicon chromosome
Accession NZ_CP054236
Organism Escherichia coli strain EcPF5

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HR070_RS21230 Protein ID WP_000244781.1
Coordinates 4448578..4448985 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HR070_RS21235 Protein ID WP_000354046.1
Coordinates 4448966..4449232 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR070_RS21210 4444535..4446268 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
HR070_RS21215 4446274..4446984 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HR070_RS21220 4447009..4447905 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HR070_RS21225 4448017..4448538 + 522 WP_001055874.1 flavodoxin FldB -
HR070_RS21230 4448578..4448985 - 408 WP_000244781.1 protein YgfX Toxin
HR070_RS21235 4448966..4449232 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HR070_RS21240 4449475..4450455 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
HR070_RS21245 4450532..4451191 - 660 WP_000250274.1 hemolysin III family protein -
HR070_RS21250 4451355..4451666 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HR070_RS21255 4451711..4453144 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T159182 WP_000244781.1 NZ_CP054236:c4448985-4448578 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159182 NZ_CP069973:c4372874-4372623 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT159182 WP_000354046.1 NZ_CP054236:c4449232-4448966 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159182 NZ_CP069973:c4372626-4372372 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References