Detailed information of TA system TA159181

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4156131..4156825
• Replicon: chromosome
• Accession: NZ_CP054236
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: HR070_RS19995
• Protein ID: WP_001094398.1
• Coordinates: 4156131..4156499 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HR070_RS20000
• Protein ID: WP_077249034.1
• Coordinates: 4156520..4156825 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS19985	4154515..4155009	+	495	WP_001059463.1	MarR family transcriptional regulator	-
HR070_RS19990	4155444..4155782	-	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
HR070_RS19995	4156131..4156499	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
HR070_RS20000	4156520..4156825	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR070_RS20005	4156929..4157573	-	645	WP_148458590.1	antitoxin of toxin-antitoxin stability system	-
HR070_RS20010	4157592..4157813	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HR070_RS20015	4157882..4158358	-	477	WP_001424026.1	RadC family protein	-
HR070_RS20020	4158374..4158847	-	474	WP_000855059.1	antirestriction protein	-
HR070_RS20025	4159189..4160010	-	822	WP_176324891.1	DUF945 domain-containing protein	-
HR070_RS20030	4160110..4160343	-	234	WP_001213776.1	DUF905 family protein	-
HR070_RS20035	4160461..4160913	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	4110213..4157573	47360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T159181 WP_001094398.1 NZ_CP054236:c4156499-4156131 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T159181 NZ_CP069973:c4372626-4372372 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT159181 WP_077249034.1 NZ_CP054236:c4156825-4156520 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT159181 NZ_CP069973:c4372874-4372623 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4