Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3971689..3972307 Replicon chromosome
Accession NZ_CP054236
Organism Escherichia coli strain EcPF5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HR070_RS19135 Protein ID WP_001291435.1
Coordinates 3972089..3972307 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HR070_RS19130 Protein ID WP_000344800.1
Coordinates 3971689..3972063 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR070_RS19120 3966779..3967972 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HR070_RS19125 3967995..3971144 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
HR070_RS19130 3971689..3972063 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HR070_RS19135 3972089..3972307 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HR070_RS19140 3972480..3973031 + 552 WP_000102539.1 maltose O-acetyltransferase -
HR070_RS19145 3973147..3973617 + 471 WP_001304825.1 YlaC family protein -
HR070_RS19150 3973781..3975331 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HR070_RS19155 3975373..3975726 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HR070_RS19165 3976105..3976416 + 312 WP_000409908.1 MGMT family protein -
HR070_RS19170 3976447..3977019 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159180 WP_001291435.1 NZ_CP054236:3972089-3972307 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T159180 NZ_CP054236:3972089-3972307 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT159180 WP_000344800.1 NZ_CP054236:3971689-3972063 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT159180 NZ_CP054236:3971689-3972063 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References