Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2193604..2194435 Replicon chromosome
Accession NZ_CP054236
Organism Escherichia coli strain EcPF5

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HR070_RS10370 Protein ID WP_000854814.1
Coordinates 2193604..2193978 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag HR070_RS10375 Protein ID WP_001285586.1
Coordinates 2194067..2194435 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR070_RS10330 2189000..2190166 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HR070_RS10335 2190285..2190758 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
HR070_RS10340 2190956..2192014 + 1059 WP_001200890.1 FUSC family protein -
HR070_RS10345 2192186..2192515 + 330 WP_000450409.1 DUF496 family protein -
HR070_RS10350 2192616..2192798 - 183 WP_001016348.1 hypothetical protein -
HR070_RS10355 2192852..2192998 + 147 Protein_2023 transposase domain-containing protein -
HR070_RS10360 2193287..2193400 - 114 WP_001161660.1 DUF957 domain-containing protein -
HR070_RS10365 2193413..2193607 - 195 WP_000988601.1 hypothetical protein -
HR070_RS10370 2193604..2193978 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
HR070_RS10375 2194067..2194435 - 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
HR070_RS10380 2194509..2194730 - 222 WP_000692323.1 DUF987 domain-containing protein -
HR070_RS10385 2194799..2195275 - 477 WP_001351157.1 RadC family protein -
HR070_RS10390 2195291..2195776 - 486 WP_000213703.1 antirestriction protein -
HR070_RS10395 2195867..2196688 - 822 WP_001234569.1 DUF945 domain-containing protein -
HR070_RS10400 2196909..2197319 - 411 WP_000846704.1 hypothetical protein -
HR070_RS10405 2197335..2198012 - 678 WP_001362823.1 hypothetical protein -
HR070_RS10410 2198148..2199218 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T159168 WP_000854814.1 NZ_CP054236:c2193978-2193604 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T159168 NZ_CP069973:c1945579-1945322 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT159168 WP_001285586.1 NZ_CP054236:c2194435-2194067 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT159168 NZ_CP069973:c1945310-1944765 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References