Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4113402..4114020 Replicon chromosome
Accession NZ_CP054232
Organism Escherichia coli strain EcPF7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HR071_RS19745 Protein ID WP_001291435.1
Coordinates 4113402..4113620 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HR071_RS19750 Protein ID WP_000344800.1
Coordinates 4113646..4114020 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR071_RS19710 4108689..4109261 + 573 WP_000779831.1 YbaY family lipoprotein -
HR071_RS19715 4109292..4109603 - 312 WP_000409908.1 MGMT family protein -
HR071_RS19725 4109982..4110335 + 354 WP_000878135.1 DUF1428 family protein -
HR071_RS19730 4110377..4111927 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HR071_RS19735 4112091..4112561 - 471 WP_000136192.1 YlaC family protein -
HR071_RS19740 4112677..4113228 - 552 WP_000102539.1 maltose O-acetyltransferase -
HR071_RS19745 4113402..4113620 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HR071_RS19750 4113646..4114020 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HR071_RS19755 4114566..4117715 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
HR071_RS19760 4117738..4118931 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159150 WP_001291435.1 NZ_CP054232:c4113620-4113402 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T159150 NZ_CP069970:1789-2205 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT159150 WP_000344800.1 NZ_CP054232:c4114020-4113646 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT159150 NZ_CP069970:1562-1792 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References