Detailed information of TA system TA159142

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3229475..3230307
• Replicon: chromosome
• Accession: NZ_CP054232
• Organism: Escherichia coli strain EcPF7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: HR071_RS15455
• Protein ID: WP_000854753.1
• Coordinates: 3229475..3229849 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: HR071_RS15460
• Protein ID: WP_001540478.1
• Coordinates: 3229939..3230307 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR071_RS15435	3225828..3227366	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
HR071_RS15440	3228115..3228684	-	570	WP_001560692.1	DUF4942 domain-containing protein	-
HR071_RS15445	3228781..3228978	-	198	WP_000839281.1	DUF957 domain-containing protein	-
HR071_RS15450	3228990..3229478	-	489	WP_000777545.1	hypothetical protein	-
HR071_RS15455	3229475..3229849	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
HR071_RS15460	3229939..3230307	-	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR071_RS15465	3230470..3230691	-	222	WP_000692311.1	DUF987 domain-containing protein	-
HR071_RS15470	3230754..3231230	-	477	WP_001186786.1	RadC family protein	-
HR071_RS15475	3231246..3231731	-	486	WP_000214398.1	antirestriction protein	-
HR071_RS15480	3231822..3232640	-	819	WP_001773857.1	DUF945 domain-containing protein	-
HR071_RS15485	3232730..3232963	-	234	WP_001278283.1	DUF905 family protein	-
HR071_RS15490	3232969..3233646	-	678	WP_001097301.1	hypothetical protein	-
HR071_RS15495	3233794..3234474	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX	3202427..3285394	82967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T159142 WP_000854753.1 NZ_CP054232:c3229849-3229475 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T159142 NZ_CP069968:c3327453-3326920 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT159142 WP_001540478.1 NZ_CP054232:c3230307-3229939 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT159142 NZ_CP069968:c3327730-3327464 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68